02256nas a2200217 4500008004100000245015600041210006900197300001200266490000700278520155000285653001801835653001601853653002701869653001301896100001901909700001701928700001801945700002001963700001401983856004101997 2021 eng d00aA New Leaf Litter Toad of Leptobrachella Smith, 1925 (Anura, Megophryidae) from Sichuan Province, China with Supplementary Description of L. oshanensis0 aNew Leaf Litter Toad of Leptobrachella Smith 1925 Anura Megophry a143-1660 v123 a

A new species of the Asian leaf litter toad genus Leptobrachella is described from Sichuan Province, China. Molecular phylogenetic analyses based on mitochondrial and nuclear gene sequences clustered the new species as an independent clade nested into L. oshanensis species group. The new species could be distinguished from its congeners by a combination of following characters: body size moderate (25.8–32.6 mm in male, 33.7–34.1 mm in female); distinct black spots present on flanks; toes rudimentary webbed, with narrow lateral fringes, dermal ridges under toes interrupted at articulations; ventral belly cream white with variable brown specking; skin on dorsum relatively smooth with fine tiny granules or short ridges; iris copper above, silver bellow; greyish black patches on posterior thigh absent or small; spines on surface of chest absent in male during breeding season; nasals entirely or partially separated from sphenethmoid in male; dorsal surface of tadpoles semitransparent light brown, spots on tail absent, keratodont row formula I: 3+3(2+2)/2+2: I; calls simple, call series basically consist of repeated long calls, at dominant frequency(4831.9 ± 155.8)Hz and call duration(544.5 ± 146.8)ms. In addition, we made supplementary description on L. oshanensis including holotype, variations, tadpoles, skull and bioacoustics. Besides, this paper reports cases of femoral adipose glands in the genus Leptobrachella as first known sexual dimorphism skin glands for males of Megophryidae.

10amultiple data10anew species10asympatric distribution10ataxonomy1 aSHI, Shengchao1 aHOU, Yinmeng1 aSONG, Zhaobin1 aJiang, Jianping1 aWang, Bin uhttps://bio.acousti.ca/es/node/5842901534nas a2200181 4500008004100000245015100041210006900192520087300261653001301134653001001147653001701157653002301174653001301197100003101210700001901241700001601260856007601276 2019 eng d00aTaxonomic status of topotypic varieties of Scinax x-signatus species assigned by Spix (1824) for the Brazilian Amazon (Anura, Hylidae, Scinaxinae)0 aTaxonomic status of topotypic varieties of Scinax xsignatus spec3 a

Scinax x-signatus was originally described by Spix (1824) from “ProvinciaBahiae”. The holotype was lost during World War II and a neotype was not designated, thus leading to the synonymy of S. x-signatus to Hyla affinis and Hyla coerulea, both from Amazonas. Herein we present the phenotypic variation of morphological and morphometric characters of populations from states of Bahia and Amazonas. Additionally we provide the bioacoustic variation from state of Bahia. Bioacoustic results show an acoustically cohesive population in Bahia, however morphometric and morphological results show that these populations may not represent the same species. Our combined results show that Bahia populations of S. x-signatus are significantly different from population of Amazonas and a more comprehensive study with molecular data should corroborate that.

10aAmazonas10aBahia10abioacoustics10aScinax ruber clade10ataxonomy1 aSichieri, Guilherme, R. F.1 aGordo, Marcelo1 aNunes, Ivan uhttp://periodicos.unisanta.br/index.php/ENPG/article/viewFile/2087/158501494nas a2200217 4500008004100000245012600041210006900167520068500236653003900921653002400960653001600984653001901000653001301019100002001032700001701052700002101069700002401090700001701114700002201131856012301153 2019 eng d00aA new species of Megophrys Kuhl & Van Hasselt (Amphibia: Megophryidae) from Borneo allied to M. nasuta (Schlegel, 1858)0 anew species of Megophrys Kuhl Van Hasselt Amphibia Megophryidae 3 a

Within Sundaland, the species diversity of the genus Megophrys is richest in Borneo, with some species restricted to the northern highlands of the island. On the basis of molecular, morphological and acoustic evidence, we describe a new species of large-sized Megophrys, once confused with M. nasuta. The new species Megophrys kalimantanensis sp. nov. can be differentiated from all its congeners by large genetic distance more than 6.11% in the mitochondrial 16S rRNA gene, much shorter rostral and orbital appendages, and unique advertisement call. This new species has been recorded from medium to high altitudes of mountain forest in southern and northernmost Borneo.

10aMegophrys kalimantanensis sp. nov.10amolecular phylogeny10anew species10aSoutheast Asia10ataxonomy1 aMUNIR, MISBAHUL1 aHAMIDY, AMIR1 aMatsui, Masafumi1 aISKANDAR, DJOKO, T.1 aSIDIK, IRVAN1 aSHIMADA, TOMOHIKO uhttps://www.biotaxa.org/Zootaxa/issue/view/zootaxa.4679.1https://www.biotaxa.org/Zootaxa/article/view/zootaxa.4679.1.102264nas a2200241 4500008004100000022001400041245010400055210006900159260001600228300000800244490000900252520147400261653001301735653002901748653001501777653002301792653002401815653001301839100001901852700001901871700001601890856011601906 2017 eng d a1175-532600aA new species of smooth-skinned Spinomantis frog (Anura: Mantellidae) from south-eastern Madagascar0 anew species of smoothskinned Spinomantis frog Anura Mantellidae  cApr-09-2017 a3790 v43173 a

We present molecular evidence for the presence of two species morphologically similar to Spinomantis bertini in Andohahela National Park, south-eastern Madagascar, differing by 5.5−6.3% pairwise DNA sequence divergences in the mitochondrial 16S rRNA gene. One of these was observed at higher elevations of ca. 1650 m above sea level, whereas the other was found at lower elevations of ca. 715 m a.s.l., close to the type locality of S. bertini (Isaka-Ivondro), and in one other location (Andreoky, ca. 1050 a.s.l.). We herein assign these low- to mid-elevation specimens to S. bertini based on their occurrence near the type locality and general agreement in colour pattern with the type specimen of Gephyromantis bertini Guibé, 1947. The high-elevation form is described as Spinomantis beckei sp. nov. based on its molecular divergence and reciprocal monophyly with respect to S. bertini, lower expression of greenish dorsal colour and less distinct frenal stripe. Based on a comparison of published call descriptions for S. bertini and our recordings of S. beckei, we hypothesize that S. bertini has a lower note repetition rate in advertisement calls. Molecular data suggest that the S. bertini species complex is more diverse than previously recognized, with at least two more candidate species identified: S. sp. Ca7 from Ranomafana National Park, and a newly identified candidate species S. sp. Ca12 from Pic d’Ivohibe Special Reserve.

10aAmphibia10aAndohahela National Park10aMadagascar10aSpinomantis beckei10aSpinomantis bertini10ataxonomy1 aVences, Miguel1 aKöhler, Jörn1 aGlaw, Frank uhttps://biotaxa.org/Zootaxa/issue/view/zootaxa.4317.2https://biotaxa.org/Zootaxa/article/view/zootaxa.4317.2.1202753nas a2200229 4500008004100000022001400041245014000055210006900195260001600264300001400280490000800294520198400302653002502286653001702311653001702328653002402345653001302369100002802382700003302410700002602443856005402469 2017 eng d a1313-298900aThe acoustic repertoire of the Atlantic Forest Rocket Frog and its consequences for taxonomy and conservation (Allobates, Aromobatidae)0 aacoustic repertoire of the Atlantic Forest Rocket Frog and its c cSep-08-2018 a141 - 1530 v6923 a

The use of acoustic signals is a common characteristic of most anuran species to mediate intraspecific communication. Besides many social purposes, one of the main functions of these signals is species recognition. For this reason, this phenotypic trait is normally applied to taxonomy or to construct evolutionary relationship hypotheses. Here the acoustic repertoire of five populations of the genus Allobates from the Brazilian Atlantic Forest are presented for the first time, on a vulnerable to extinction Neotropical taxon. The description of males’ advertisement and aggressive calls and a female call emitted in a courtship context are presented. In addition, the advertisement calls of individuals from distinct geographical regions were compared. Differences in frequency range and note duration may imply in taxonomic rearrangements of these populations, once considered distinct species, and more recently, proposed as a single species, Allobates olfersioides. Calls of the male from the state of Rio de Janeiro do not overlap spectrally with calls of males from northern populations, while the shorter notes emitted by males from Alagoas also distinguishes this population from the remaining southern populations. Therefore, it is likely that at least two of the junior synonyms should be revalidated. Similarities among male advertisement and female calls are generally reported in other anuran species; these calls may have evolved from a preexisting vocalization common to both sexes. Male aggressive calls were different from both the male advertisement and female calls, since it was composed by a longer and multi-pulsed note. Aggressive and advertisement calls generally have similar dominant frequencies, but they have temporal distinctions. Such patterns were corroborated with the Atlantic Forest Rocket Frogs. These findings may support future research addressing the taxonomy of the group, behavioral evolution, and amphibian conservation.

10aAnuran communication10abioacoustics10aconservation10afemale vocalization10ataxonomy1 aForti, Lucas, Rodriguez1 ada Silva, Thaís, Renata Áv1 aToledo, Luís, Felipe uhttps://zookeys.pensoft.net/articles.php?id=1218704329nas a2200409 4500008004100000022001400041245011500055210006900170260001600239300000600255490000900261520318800270653001303458653001003471653000903481653001803490653002103508653001903529653001603548653000903564653001003573653001003583653002503593653001303618653001703631100001903648700001903667700002203686700002503708700002603733700001803759700001603777700002603793700002403819700001903843856005703862 2017 eng d a1175-532600aThe use of bioacoustics in anuran taxonomy: theory, terminology, methods and recommendations for best practice0 ause of bioacoustics in anuran taxonomy theory terminology method cNov-04-2017 a10 v42513 a

Vocalizations of anuran amphibians have received much attention in studies of behavioral ecology and physiology, but also provide informative characters for identifying and delimiting species. We here review the terminology and variation of frog calls from a perspective of integrative taxonomy, and provide hands-on protocols for recording, analyzing, comparing, interpreting and describing these sounds. Our focus is on advertisement calls, which serve as premating isolation mechanisms and, therefore, convey important taxonomic information. We provide recommendations for terminology of frog vocalizations, with call, note and pulse being the fundamental subunits to be used in descriptions and comparisons. However, due to the complexity and diversity of these signals, an unequivocal application of the terms call and note can be challenging. We therefore provide two coherent concepts that either follow a note-centered approach (defining uninterrupted units of sound as notes, and their entirety as call) or a call-centered approach (defining uninterrupted units as call whenever they are separated by long silent intervals) in terminology. Based on surveys of literature, we show that numerous call traits can be highly variable within and between individuals of one species. Despite idiosyncrasies of species and higher taxa, the duration of calls or notes, pulse rate within notes, and number of pulses per note appear to be more static within individuals and somewhat less affected by temperature. Therefore, these variables might often be preferable as taxonomic characters over call rate or note rate, which are heavily influenced by various factors. Dominant frequency is also comparatively static and only weakly affected by temperature, but depends strongly on body size. As with other taxonomic characters, strong call divergence is typically indicative of species-level differences, whereas call similarities of two populations are no evidence for them being conspecific. Taxonomic conclusions can especially be drawn when the general advertisement call structure of two candidate species is radically different and qualitative call differences are thus observed. On the other hand, quantitative differences in call traits might substantially vary within and among conspecific populations, and require careful evaluation and analysis. We provide guidelines for the taxonomic interpretation of advertisement call differences in sympatric and allopatric situations, and emphasize the need for an integrative use of multiple datasets (bio-acoustics, morphology, genetics), particularly for allopatric scenarios. We show that small-sized frogs often emit calls with frequency components in the ultrasound spectrum, although it is unlikely that these high frequencies are of biological relevance for the majority of them, and we illustrate that detection of upper harmonics depends also on recording distance because higher frequencies are attenuated more strongly. Bioacoustics remains a prime approach in integrative taxonomy of anurans if uncertainty due to possible intraspecific variation and technical artifacts is adequately considered and acknowledged.

10aAmphibia10aAnura10acall10acall analysis10acall description10acall variation10adefinitions10anote10apulse10asound10aspecies delimitation10ataxonomy10avocalization1 aKöhler, Jörn1 aJansen, Martin1 aRodríguez, Ariel1 aKok, Philippe, J. R.1 aToledo, Luís, Felipe1 aEmmrich, Mike1 aGlaw, Frank1 aHaddad, Célio, F. B.1 aRödel, Mark-Oliver1 aVences, Miguel uhttp://biotaxa.org/Zootaxa/issue/view/zootaxa.4251.1