02700nas a2200229   4500008004100000022001400041245013400055210006900189260002000258300001400278490000700292520196200299653002302261653001702284653001502301653001502316100001902331700001602350700001502366700002002381856006902401       2016                        eng d  a0046-225X00aThe Complete Mitochondrial Genome and Song Evolution of the Monotypic Genus <i>U.</i> Tarbinsky, 1932 (Orthoptera: Tettigoniidae)0 aComplete Mitochondrial Genome and Song Evolution of the Monotypi  cOct-19-20162019  a737 - 7460 v453 a<p>The insect Uvarovites inflatus Uvarov is highly appreciated in China. It is known for its distinctive songs and horn-like forewings and is raised commercially for insect lovers. U. inflatus was previously categorized as part of the mono- typic genus Uvarovites; however, there was little molecular evidence to support this taxonomic classification. This study obtained and investigated the mitogenome of U. inflatus, and its songs were characterized and compared with other Ensifera species whose mitogenomes are available. By performing the mitochondrial analysis, we were able to assess the phylogenetic relationships between these species and discuss the evolution of Ensifera calling songs. The mitogenome of U. inflatus is 15,956bp in length and contains 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region. The organization and orientation of the U. inflatus mitogenome are similar to those of other Tettigonioidea species. Phylogenetic analysis based on 13 protein-coding genes showed that the superfamily Tettigonioidea is monophyletic, as are the other six tested subfamilies from Tettigonioidea. Our results also indicated that Grylloidea is monophyletic. A Bayesian relaxed clock analysis showed that the differentiation of U. inflatus and Gampsocleis gratiosa Brunner occurred in the middle Miocene, suggesting that their speciation occurred over a long evolutionary period. The results provide significant support for the establishment of the monotypic genus Uvarovites. Calling song analysis showed that at least two discrete steps of independent evolution occurred during the change from pure tone to broadband noise, and that the ancestor of existing Ensifera was more likely to have emitted pure-tone songs than broadband signals. Together, the mitogenome, molecular clock, and acoustic data allowed us to clarify the taxonomic state of U. inflatus and propose a timeline for the evolution of Ensifera songs.</p>
10aadaptive evolution10ainsect songs10amitogenome10aOrthoptera1 aWang, Yinliang1 aZhao, Hanbo1 aZhang, Xue1 aRen, Bing-Zhong  uhttps://academic.oup.com/ee/article-lookup/doi/10.1093/ee/nvw031