02386nas a2200229   4500008004100000245010400041210006900145520161400214653001401828653001001842653002201852653002901874653002201903653000901925100001701934700001801951700002301969700001401992700001602006700001402022856012002036       2013                        eng d00aMechanical tuning of the moth ear: distortion-product otoacoustic emissions and tympanal vibrations0 aMechanical tuning of the moth ear distortionproduct otoacoustic 3 a<p>The mechanical tuning of the ear in the moth Empyreuma pugione was investigated by distortion-product otoacoustic emissions (DPOAE) and laser Doppler vibrometry (LDV). DPOAE audiograms were assessed using a novel protocol that may be advantageous for non-invasive auditory studies in insects. To evoke DPOAE, two-tone stimuli within frequency and level ranges that generated a large matrix of values (960 frequency&ndash;level combinations) were used to examine the acoustic space in which the moth tympanum shows its best mechanical and acoustical responses. The DPOAE tuning curve derived from the response matrix resembles that obtained previously by electrophysiology, and is V-shaped and tuned to frequencies between 25 and 45 kHz with low Q10dB values of 1.21&plusmn;0.26. In addition, while using a comparable stimulation regime, mechanical distortion in the displacement of the moth&rsquo;s tympanal membrane at the stigma was recorded with a laser Doppler vibrometer. The corresponding mechanical vibration audiograms were compared with DPOAE audiograms. Both types of audiograms have comparable shape, but most of the mechanical response fields are shifted towards lower frequencies. We showed for the first time in moths that DPOAE have a pronounced analogy in the vibration of the tympanic membrane where they may originate. Our work supports previous studies that point to the stigma (and the internally associated transduction machinery) as an important place of sound amplification in the&nbsp;moth ear, but also suggests a complex mechanical role for the rest of the transparent zone.</p>
10aAudiogram10aDPOAE10aEmpyreuma pugione10alaser Doppler vibrometry10amechanical tuning10amoth1 aMora, E., C.1 aCobo-Cuan, A.1 aMacias-Escriva, F.1 aPerez, M.1 aNowotny, M.1 aKossl, M.  uhttp://jeb.biologists.org/cgi/doi/10.1242/jeb.085902https://syndication.highwire.org/content/doi/10.1242/jeb.085902