Phylogeny of the cricket subfamily Eneopterinae (Orthoptera, Grylloidea, Eneopteridae) based on four molecular loci and morphology

The phylogenetic relationships of 39 species of Eneopterinae crickets are reconstructed using four molecular markers (16S rRNA, 12S rRNA, cytochrome b , 18S rRNA) and a large morphological data set. Phylogenetic analysis via direct optimisation of DNA sequence data using parsimony as optimality criterion is done for six combinations of weighting parameter sets in a sensitivity analysis . The results are discussed in a twofold purpose: W rst, in term of signi W cance of the molecular markers for phylogeny reconstruction in Ensifera, as our study represents the W rst molecular phylogeny performed for this insect suborder at this level of diversity; second, in term of corrobora- tion of a previous phylogeny of Eneopterinae, built on morphological data alone. The four molecular markers all convey phylogen etic signal, although variously distributed on the tree. The monophyly of the subfamily, that of three over W ve tribes, and of 10 over 13 genera, are recovered. Finally, previous hypotheses on the evolution of acoustic devices and signals in the Eneopterinae clade are brie X y tested, and supported, by our new data set.