03024nas a2200265 4500008004100000245014600041210006900187520194300256653002702199653002502226653001702251653001802268653004002286653001502326100002002341700001902361700002002380700001802400700001702418700002102435700002502456700002302481700001602504856023802520 2019 eng d00aVocal divergence is concordant with genomic evidence for strong reproductive isolation in grasshopper mice ( Onychomys )0 aVocal divergence is concordant with genomic evidence for strong 3 a

Behavioral barriers to gene flow often evolve faster than intrinsic incompatibilities and can eliminate the opportunity for hybridization between interfertile species. While acoustic signal divergence is a common driver of premating isolation in birds and in‐ sects, its contribution to speciation in mammals is less studied. Here we characterize the incidence of, and potential barriers to, hybridization among three closely related species of grasshopper mice (genus Onychomys). All three species use long‐distance acoustic signals to attract and localize mates; Onychomys arenicola and Onychomys torridus are acoustically similar and morphologically cryptic whereas Onychomys leu‐ cogaster is larger and acoustically distinct. We used genotyping‐by‐sequencing (GBS) to test for evidence of introgression in 227 mice from allopatric and sympatric lo‐ calities in the western United States and northern Mexico. We conducted laboratory mating trials for all species pairs to assess reproductive compatibility, and recorded vocalizations from O. arenicola and O. torridus in sympatry and allopatry to test for evidence of acoustic character displacement. Hybridization was rare in nature and, contrary to prior evidence for O. torridus/O. arenicola hybrids, only involved O. leu‐ cogaster and O. arenicola. In contrast, laboratory crosses between O. torridus and O. arenicola produced litters whereas O. leucogaster and O. arenicola crosses did not. Call fundamental frequency in O. torridus and O. arenicola was indistinguishable in allopatry but significantly differentiated in sympatry, a pattern consistent with repro‐ ductive character displacement. These results suggest that assortative mating based on a long‐distance signal is an important isolating mechanism between O. torridus and O. arenicola and highlight the importance of behavioral barriers in determining the permeability of species boundaries.

10aacoustic communication10abehavioral isolation10acontact zone10ahybridization10areproductive character displacement10aspeciation1 aCampbell, Polly1 aArévalo, Lena1 aMartin, Heather1 aChen, Charles1 aSun, Shuzhen1 aRowe, Ashlee, H.1 aWebster, Michael, S.1 aSearle, Jeremy, B.1 aPasch, Bret uhttps://onlinelibrary.wiley.com/doi/abs/10.1002/ece3.5770https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.5770https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.5770https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ece3.577002101nas a2200181 4500008004100000022001400041245016200055210006900217260001600286520134800302653002701650653002101677653002101698653001501719100002401734700002701758856013401785 2017 eng d a0024-406600aSubspecies-specific song preferences and diverged heterospecific discrimination in females of the bush-cricket Isophya kraussii (Orthoptera: Phaneropterinae)0 aSubspeciesspecific song preferences and diverged heterospecific  cSep-09-20173 a

Divergence of the acoustic signals used in mate calling may be an important driver of speciation. Male song may vary accordingly to, in mismatch with or independently of female preferences. Therefore, to estimate the importance of male signal variation between subspecies, female preferences against subspecies-specific signal variants must be tested. We examined the female response probability in the two subspecies of the bush-cricket Isophya kraussii for consubspecific and heterosubspecific male signals, and also for the song of a closely related species (I. camptoxypha), which is sympatric with one, but allopatric to the other subspecies. Performing no-choice playback experiments, we found that females of both subspecies responded to the male song of their own subspecies with significantly higher probability than to heterosubspecific and heterospecific songs. Response specificity for consubspecific vs. heterosubspecific signals was not significantly asymmetric comparing the two subspecies. A significant difference was found, however, in the discrimination of heterospecific calls: females showed stronger discrimination against the song of I. camptoxypha in the subspecies sympatric with that species. Our results are best explained by a tightly covarying, stepwise coevolution of male signals and female preferences.

10aacoustic communication10amate recognition10asignal evolution10aspeciation1 aOrci, Kirill, Márk1 aIorgu, Ionuț, Ștefan uhttp://academic.oup.com/biolinnean/article/doi/10.1093/biolinnean/blx094/4110201/Subspeciesspecific-song-preferences-and-diverged02511nas a2200253 4500008004100000022001300041245012200054210006900176260001600245300001200261490000800273520169800281653002701979653001502006653002202021653002102043653002702064653002102091653001502112653001702127100002302144700002402167856006602191 2017 eng d a0003347200aDivergent mechanisms of acoustic mate recognition between closely related field cricket species ( Teleogryllus spp.)0 aDivergent mechanisms of acoustic mate recognition between closel cJan-08-2017 a17 - 250 v1303 a

Effective recognition of conspeci fi c mating signals reduces the risk of maladaptive hybridization. Dis- secting the signal recognition algorithms that underlie preferences is a useful approach for testing whether closely related taxa evaluate the same or different signal features to achieve mate recognition. Such data provide information about potential constraints and targets of selection during evolutionary divergence. Using a series of mate choice trials, we tested whether closely related, but genetically and phenotypically divergent, fi eld cricket species ( Teleogryllus oceanicus and Teleogryllus commodus ) use shared or distinct recognition algorithms when evaluating acoustic male calling songs. These species overlap in sympatry, show premating isolation based on female discrimination of male calling songs, yet are capable of producing hybrid offspring. Unexpectedly, female selectivity for features of male song differed between the two species. We found that the two species use a combination of shared and unique signal fi ltering mechanisms, and we characterized how information about male carrier frequency, pulse rate and temporal patterning is integrated to achieve song recognition in each species. These results illustrate how comparatively few, simple modi fi cations in key components of signal recognition algo- rithms can lead to striking interspeci fi c discrimination between closely related taxa, despite apparent signal complexity. The fi nding that some steps during signal recognition and fi ltering are shared between the species, while others differ, can help to identify behavioural traits targeted by selection during evolutionary divergence

10aacoustic communication10adivergence10afemale preference10amate recognition10areproductive isolation10asexual selection10aspeciation10aTeleogryllus1 aBailey, Nathan, W.1 aHennig, R. Matthias uhttp://linkinghub.elsevier.com/retrieve/pii/S0003347217301823