02700nas a2200229 4500008004100000022001400041245013400055210006900189260002000258300001400278490000700292520196200299653002302261653001702284653001502301653001502316100001902331700001602350700001502366700002002381856006902401 2016 eng d a0046-225X00aThe Complete Mitochondrial Genome and Song Evolution of the Monotypic Genus U. Tarbinsky, 1932 (Orthoptera: Tettigoniidae)0 aComplete Mitochondrial Genome and Song Evolution of the Monotypi cOct-19-20162019 a737 - 7460 v453 a

The insect Uvarovites inflatus Uvarov is highly appreciated in China. It is known for its distinctive songs and horn-like forewings and is raised commercially for insect lovers. U. inflatus was previously categorized as part of the mono- typic genus Uvarovites; however, there was little molecular evidence to support this taxonomic classification. This study obtained and investigated the mitogenome of U. inflatus, and its songs were characterized and compared with other Ensifera species whose mitogenomes are available. By performing the mitochondrial analysis, we were able to assess the phylogenetic relationships between these species and discuss the evolution of Ensifera calling songs. The mitogenome of U. inflatus is 15,956bp in length and contains 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region. The organization and orientation of the U. inflatus mitogenome are similar to those of other Tettigonioidea species. Phylogenetic analysis based on 13 protein-coding genes showed that the superfamily Tettigonioidea is monophyletic, as are the other six tested subfamilies from Tettigonioidea. Our results also indicated that Grylloidea is monophyletic. A Bayesian relaxed clock analysis showed that the differentiation of U. inflatus and Gampsocleis gratiosa Brunner occurred in the middle Miocene, suggesting that their speciation occurred over a long evolutionary period. The results provide significant support for the establishment of the monotypic genus Uvarovites. Calling song analysis showed that at least two discrete steps of independent evolution occurred during the change from pure tone to broadband noise, and that the ancestor of existing Ensifera was more likely to have emitted pure-tone songs than broadband signals. Together, the mitogenome, molecular clock, and acoustic data allowed us to clarify the taxonomic state of U. inflatus and propose a timeline for the evolution of Ensifera songs.

10aadaptive evolution10ainsect songs10amitogenome10aOrthoptera1 aWang, Yinliang1 aZhao, Hanbo1 aZhang, Xue1 aRen, Bing-Zhong uhttps://academic.oup.com/ee/article-lookup/doi/10.1093/ee/nvw03101637nas a2200205 4500008004100000245014200041210006900183300001200252490000700264520097800271653001701249653001201266653001801278653002001296100001901316700001601335700001901351700002001370856004101390 2011 eng d00aAcoustic and Molecular Differentiation between Macropters and Brachypters of Eobiana engelhardti engelhardti (Orthoptera: Tettigonioidea)0 aAcoustic and Molecular Differentiation between Macropters and Br a636-6440 v503 aThis study focused on the wing dimorphism of Eobiana engelhardti engelhardti (Uvarov 1926). To examine acoustic differences between macropters and brachypters, we recorded and analyzed the calling songs of the 2 forms. Moreover, the vocal organs of E. e. engelhardti were also observed under optical and scanning electric microscopy. As a result, there were 3 “dynamic” song traits which had signi cant differences between the 2 forms, but no obvious differences were observed in vocal organs. For macropters, we assumed that differentiation of these calling songs showed compensation for a reproductive disadvantage. Finally, some molecular biology experiments were conducted to examine differences between the 2 forms at the molecular biology level; as a result, brachypterous insects were grouped in a clade, while macropterous insects were grouped into another, which indicates that these molecular differences had already occurred before these macropters migrated.10ainsect songs10akatydid10atoothed files10awing dimorphism1 aWang, Yinliang1 aZhang, Jian1 aLi, Xiao-Qiang1 aRen, Bing-Zhong uhttps://bio.acousti.ca/nl/node/4779002125nas a2200241 4500008004100000022001400041245011600055210006900171260001600240300000800256490000900264520137700273653001401650653001501664653003301679653002701712100001801739700001501757700001501772700001901787700002001806856005701826 2016 eng d a1175-532600aMicromorphological differentiation of left and right stridulatory apparatus in crickets (Orthoptera: Gryllidae)0 aMicromorphological differentiation of left and right stridulator cDec-06-2017 a5530 v41273 a

The present study compared micromorphological differentiation of stridulatory apparatus between the functional right tegmen and non-functional left tegmen, analyzed under scanning electron microscope (SEM), among eight Gryllidae species. The results showed that the main differences were found on the length and shape of files and teeth. The length of stridulatory file and teeth number were lower on the left stridulatory apparatus than that on right stridulatory apparatus in all species. However, the ratio between the length of stridulatory teeth and the interval of stridulatory teeth was significantly higher on the left stridulatory apparatus than that on right stridulatory apparatus in Dianemobius fascipes, Polionemobius taprobanensis, Pteronemobius gifuensis, Teleogryllus occipitalis and Oecanthus longicauda. In addition, the length of stridulatory teeth was positively related to number of stridulatory teeth, however, the interval of stridulatory teeth was negatively related to the ratio between the length of stridulatory teeth and the interval of stridulatory teeth for left and right stridulatory apparatus. Our result illustrated that the length of left and right stridulatory file and teeth length could be an effective character to distinguish species. Left stridulatory apparatus was not entirely degraded than right stridulatory apparatus.

10aGryllidae10aOrthoptera10ascanning electron microscope10astridulatory apparatus1 aLI, XIAOQIANG1 aZhang, Xue1 aLuo, Wenqi1 aWang, Yinliang1 aRen, Bing-Zhong uhttp://biotaxa.org/Zootaxa/issue/view/zootaxa.4127.301888nas a2200265 4500008004100000022001400041245012100055210006900176260001600245300001400261490000800275520111700283653001401400653000901414653001901423653001501442653001501457100001501472700001401487700001701501700001301518700001901531700002001550856005201570 2015 eng d a1313-298900aAcoustic, genetic and morphological variations within the katydid Gampsocleis sedakovii (Orthoptera, Tettigonioidea)0 aAcoustic genetic and morphological variations within the katydid cFeb-10-2017 a105 - 1210 v5293 aIn an attempt to explain the variation within this species and clarify the subspecies classification, an analysis of the genetic, calling songs, and morphological variations within the species Gampsocleis sedakovii is presented from Inner Mongolia, China. Recordings were compared of the male calling songs and analysis performed of selected acoustic variables. This analysis is combined with sequencing of mtDNA - COI and examination of morphological traits to perform cluster analyses. The trees constructed from different datasets were structurally similar, bisecting the six geographical populations studied. Based on two large branches in the analysis, the species Gampsocleis sedakovii was partitioned into two subspecies, Gampsocleis sedakovii sedakovii (Fischer von Waldheim, 1846) and Gampsocleis sedakovii obscura (Walker, 1869). Comparing all the traits, the individual of Elunchun (ELC) was the intermediate type in this species according to the acoustic, genetic, and morphological characteristics. This study provides evidence for insect acoustic signal divergence and the process of subspeciation.10aacoustics10agene10ainterim morphs10amorphology10asubspecies1 aZhang, Xue1 aWen, Ming1 aLi, Junjiain1 aZhu, Hui1 aWang, Yinliang1 aRen, Bing-Zhong uhttp://zookeys.pensoft.net/articles.php?id=6043