Taxonomic status of topotypic varieties of Scinax x-signatus species assigned by Spix (1824) for the Brazilian Amazon (Anura, Hylidae, Scinaxinae)

Scinax x-signatus was originally described by Spix (1824) from “ProvinciaBahiae”. The holotype was lost during World War II and a neotype was not designated, thus leading to the synonymy of S. x-signatus to Hyla affinis and Hyla coerulea, both from Amazonas. Herein we present the phenotypic variation of morphological and morphometric characters of populations from states of Bahia and Amazonas. Additionally we provide the bioacoustic variation from state of Bahia. Bioacoustic results show an acoustically cohesive population in Bahia, however morphometric and morphological results show that these populations may not represent the same species. Our combined results show that Bahia populations of S. x-signatus are significantly different from population of Amazonas and a more comprehensive study with molecular data should corroborate that.